Experimental Techniques

Period: First        ECTS: 6


Course contents

Activity 1 Advanced seminars

  • Electronic microscopy and characterization of protein structure. (BIO)
  • Near field microscopies (AFM). (BIO, NANO)
  • Spectroscopic techniques applied to biomolecules. (BIO, NANO)
  • Plasmon surface resonance. (BIO)
  • Fluorescence microscopy. (NANO,BIO)
  • Infrared spectroscopy. (NANO, BIO)
  • Sensors and materials. (NANO, BIO)
  • Functionalization of surfaces. (BIO, NANO)

Activity 2 Practical labs

  • Superconductivity and cryogenics. (CONDMAT)
  • Magnetism. (NANO)
  • Graphene. (NANO)
  • Surface growth. (NANO)
  • Infrared and ultraviolet spectroscopy. (NANO, BIO)
  • Fluorescence spectroscopy. (NANO, BIO)
  • Plasmon surface resonance. (BIO)
  • Atomic force microscopy in liquids. (NANO, BIO)

Bibliography

  • Estructura de proteínas. Gómez-Moreno Calera, Sancho Sanz. Ariel Ciencia. 2003.
  • Principles of Fluorescence Spectroscopy. Joseph R. Lakowicz
  • BIOQUÍMICA, Stryer (2003) Ed. Reverté. 5ª Ed. p 261
  • PHYSICSL BIOCHEMISTRY, K.E. Van Holde, W.C. Johnson, P.S. Ho (2006) Prentice Hall.
  • BIOPHYSICS. An introduction. R. Cotterill (2003) Wiley
  • PHYSICAL CHEMISTRY Principles and Applications in Biological Sciences. Tinoco et al (2002) Prentice Hall.
  • ATKINS, P.W., Química Física 8ª Ed. Panamericana 2008
  • LEVINE, I.N., Fisicoquímica, Ed. Mc Graw-Hill, Madrid, 2004
  • Spectroscopic Methods For Analysis Of Protein Secondary Structure. J.T. Pelton and L.R. McLean. (2000) Analytical Biochemistry, 277, 167-176.
  • Quantitative studies of the structure of proteins in solution by Fourier-transform infrared spectroscopy. Arrondo, J.L.R. et al (1993) Prog. Biophys. Molec. Biol. 59, 33.
  • Methods for the detection and analysis of protein-protein interactions. T. Berggard, S. Linse, P. James. Proteomics (2007) 2833.
  • Isothermal Titration Calorimetry: Experimental Design, Data Analysis, ansd Probing Macromolecule/Ligand Binding and Kinetic Interactions. M.W. Freyer, E.A. Lewis, Methods in Cell Biology Vol 84 (2008) p 79
  • Spectroscopic Methods For Analysis Of Protein Secondary Structure. J.T. Pelton and -L.R. McLean. (2000) Analytical Biochemistry, 277, 167-176.


Faculty

Coordinator  Pedro J. de Pablo
Email  p.j.depablo@uam.es
Website  http://www.researchgate.net/profile/Pedro_Pablo4/


More info on the course official guide (Guía docente)