The updated ‘Guía docente’ can be found in the Faculty web page
Period: 1st (Fall semester)
EXPERIMENTAL METHODS IN MOLECULAR AND CELL BIOLOGY
Seminar 1.- Basic genetic mechanisms and information flow.
The seminar will deal with one or more of the following topics: DNA replication and reparation, transcription and translation.
Seminar 2.- Internal organization in eukaryotic cells.
The seminar will deal with one or more of the following topics: cellular organelles, synthesis, targeting and secretion of macromolecules, vesicular transport, and mechanisms of energy conversion.
Seminar 3.- Intra- and inter-cellular chemical signals.
The seminar will deal with one or more of the following topics: membrane receptors and intracellular effectors (channels, etc), second messengers (in particular Ca2+ and cAMP), intracellular cascades, regulation of genetic programs, nervous and immune systems and their disturbances.
Seminar 4.- Cell motility and shape, cell división and cell cycle.
The seminar will deal with one or more of the following topics: cytoskeleton, microfilaments, microtubules, muscle contraction, cilia nad flagella, molecular motors, cell division, cell cycle and its disturbances.
Seminar 5.- Cell-cell and cell-matrix adhesion, development and differentiation.
The seminar will deal with one or more of the following topics: cell junctions, types, composition and functions, structure and function of extracelular matrix, basal lamina, mechanisms implicated in development and their regulation, cellular differentiation, cell fate.
1. Solutions. Buffers
2. Purification of serum immunoglobulins: fractional precipitation of proteins. Ion exchange chromatography
3. Determination of protein concentration: Lowry
4. SDS-polyacrylamide gel electrophoresis
5. Culture of eukaryotic cells from different tissues. Growth and cell staining.
6. Growth of genetically transformed bacteria
7. Isolation of nucleic acids
8. Plasmid DNA digestion with endonuclease restriction enzymes
9. Separation of DNA molecules by agarose gel electrophoresis
10. Identification of amino acids in casein by thin layer chromatography (TLC).
EXPERIMENTAL METHODS IN PHYSICS:
Physics experiments designed to quantitatively study relevant physical processes in Biology: diffusion and osmosis, spectrophotometry: adsorption and emission spectra, diffraction. Quantification by photometry of cell growth. Forces and tension. Guided by the teacher, students will propose the objectives and design of the experiment, explore its theoretical foundation and analyze the resulting data.
COMPUTATIONAL METHODS IN PHYSICS:
Practical sessions of MATLAB programming (for Group 1, Biology)
Introduction to programming in MATLAB: elementary operations with matrices and arrays, representation of functions, scripts.
Numerical solution of ordinary and partial differential equations.
Applications to problems of Physics and Biology
COMPUTATIONAL METHODS IN BIOLOGY:
Programming in R and Statistics (for Group 2, Physics)
Cooper, G.M. and Hausman, R.E. 2009. The cell: a molecular approach. Sinauer. 5th Edition.
URL for info: http://www.sinauer.com/cooper5e/
NCBI online access to 2nd Ed: http://www.ncbi.nlm.nih.gov/books/NBK9839/
. Tymoczko, J., Berg, J., Stryer, L. 2010. Biochemistry: a short course. W. H. Freeman. 1st Edition.URL: http://www.whfreeman.com/catalog/Product/biochemistryashortcourse-firstedition-tymoczko
URL for online access to contents: http://bcs.whfreeman.com/tymoczko1e/default.asp?s=&n=&i=&v=&o=&ns=0&uid=0&rau=0
Brian R. Hunt. A guide to MATLAB for beginners and experienced users. Cambridge University Press, (2006).
Coordinator of Experimental Methods in Molecular and Cell Biology: Eva Richard
Coordinator of Experimental Methods in Physics: David Míguez
Computational methods lab:
Giorgio Cinacchi (email: firstname.lastname@example.org) (Group 1)
Blanca Lizarbe(email: email@example.com) (Group 2)
More info on the course official guide (Guía docente)